Laser desorption mass spectrometry for microbial DNA analysis.

Identifi cation of dermatophytes by matrix-assisted laser desorption/ionization time-of-fl ight mass spectrometry

cation of dermatophytes [3 – 5]. ITS sequencing is presently considered the gold standard for molecular identifi cation and phylogenetic analyses of dermatophytes. However, DNA sequencing is expensive and at least 2 – 3 days are required before a sequence is obtained from a culture. Matrix-assisted laser desorption/ionization time-of-fl ight mass spectrometry (MALDI-TOF MS) is now used routinel...

Dossier MALDI - TOF ■ Oligonucleotide analysis by MALDI - MS

Matrix-assisted laser desorption (MALDI) time-offlight (TOF) mass spectrometry (MS) has been successfully used for oligonucleotide sequence confirmation by mass analysis of fragments generated by concentration-dependent exonuclease digestion. This approach is particularly well-suited to the analysis of synthetic DNA due to the relatively short length of the DNA strands. Additionally, an importa...

Analysis of the Photo Conversion of Asphaltenes Using Laser Desorption Ionization Mass Spectrometry: Fragmentation, Ring Fusion, and Fullerene Formation

The conversion or photo conversion of asphaltenes to polycyclic aromatic hydrocarbons (PAH’s) promoted by a laser source is analyzed using both experimental and theoretical methods. We propose that during measurements performed at an intermediate laser power, fragmentation to afford PAH’s and ring fusion to yield fused PAH’s (FPAH’s) may occur either within molecular clusters (resin case) or wi...

Matrix-assisted laser desorption/ionization mass spectrometry of immobilized duplex DNA probes.

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry was used to analyze short DNA duplex probes with one strand immobilized on solid supports (straptavidin-coated magnetic beads or controlled pore glass beads). Only the non-immobilized strand could be detected. Partial denaturation was found when the duplex probes were mixed with 3-hydroxypicolinic acid, a...

Teaching Microbial Identification with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) and Bioinformatics Tools†